|SEARCH GENE INFO||BULK VERIFY INFO|
1. In Home page click on the site logo or the following buttons:
When accessing the AnnotatorPPI web server homepage you will see the fields for collecting (annotating) necessary external data, where you will be placing your data.
2. Enter your sequences
Fill up the form with the required data of the DNA sequence. Basically, you can paste your sequences into the text box. Otherwise, you can choose to upload a text FASTA file containing your DNA sequence.
The total limit for multiline sequence submissions is 50,000 letters.
You can get a quick help for each field by moving the mouse pointer over the field itself.
When you have entered all data, just click
3. Get your results annotating
Before annotating your sequence is aligned by 'Human BLAT Search Genome'. Depending on how complex your input sequence is, the alignment can take a few seconds but no more than one minute to be fulfilled (mainly depends on the sequence length).
To save the information to the server, click the 'Save Changes' button, to remove the data, click 'Delete' button and to upload an archive of all information to your computer, click 'Export All' button.
The exported information is packaged in a zip archive
4. Get your prospective protein-protein interactors
Click on the protein name or any ChiTaRS mutation ID to visualize the corresponding network protein-protein interactions (PPI). The following shows a network PPI for protein.
Right-click on the network to access a menu where you can re-calculate the layout of interactors (random, tree, circle, radial type) and export the network to a graphic file.
The following shows a network PPI for the mutation (AI685149).